MARC details
| 000 -LEADER |
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| fixed length control field |
nam a22 7a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
IE-CoIT |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20180326111923.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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| fixed length control field |
ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
180322s2017 ie ||||| |||| 00| 0|eng|| |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
IE-CoIT |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
THESES PRESS |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
123966 |
| Personal name |
Kruijf, Marcel de |
| Relator term |
author |
| 245 14 - TITLE STATEMENT |
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| Title |
The development of new molecular tools to detect Mycobacterium avium subspecies paratuberculosis from a bovine faecel environment by real-time PCR / |
| Statement of responsibility, etc. |
Marcel de Kruijf. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Cork : |
| Name of producer, publisher, distributor, manufacturer |
Cork Institute of Technology, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2017. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
201 pages : |
| Other physical details |
illustrations (some color), map ; |
| Dimensions |
30 cm |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
unmediated |
| Media type code |
n |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
volume |
| Carrier type code |
nc |
| Source |
rdacarrier |
| 490 #0 - SERIES STATEMENT |
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| Series statement |
Ph. D - Biological Sciences |
| 500 ## - GENERAL NOTE |
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| General note |
The slow-growing intracellular pathogen Mycobacterium Avium subspecies Paratuberculosos (MAP) causes Johne's Disease in domesticated and wild ruminants. Johne's disease inflicts severe annual economic losses to the dairy industry worldwide. This thesis is divided into five chapters that consist of a literature review chapter and four experimental chapters focusing on MAP strain genotyping and the development of novel molecular MAP detection tools using real-time PCR. Chapter 1 describes published information regarding MAP characteristics, Johne's disease, MAP genomics, available MAP detection methods, MAP epidemiology and genotyping. To efficiently control and monitor MAP infection, molecular typing of MAP strains provides extensive knowledge regarding the source and genetic diversity of MAP within a geographic location. Knowledge regarding MAP genotypes within Ireland is limited and therefore, a large collection of MAP isolates from 53 herds across the Republic of Ireland were genotyped using the MIRU-VNTR technique and described in chapter 2. Rapid detection of MAP from faeces using real-time PCR is crucial to halt the infectious spread within herds. MAP- specific multi-copy elements are attractive and sensitive molecular targets for real-time PCR assays to detect MAP from faeces. The multi-copy IS_MAP04 has never previously been evaluated as a potential novel target in MAP diagnostics. This evaluation is described in chapter 3. A further MAP-specific molecular target has been investigated and described in chapter 4. The truncation present in the mycobactin cluster within MAP was used as a novel MAP-specific target in real-time PCR assays to detect and conform MAP from faeces. The elimination of false-negative detection results is another crucial aspect in molecular MAP diagnostics. Chapter 5 describes the development of two novel MAP DNA extraction and real-time PCR internal controls using Mycobacterium smegmatis as a model mycobacterial strain. These developed internal controls could aide in the elimination of false-negative MAP detection results from faeces - (Abstract) |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis |
| Degree type |
|
| Name of granting institution |
Cork Institute of Technology, |
| Year degree granted |
2017. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
93631 |
| Topical term or geographic name entry element |
Mycobacterium avium paratuberculosis |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
45372 |
| Topical term or geographic name entry element |
Polymerase chain reaction |
| General subdivision |
Diagnostic use |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
45298 |
| Topical term or geographic name entry element |
Molecular epidemiology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
35555 |
| Topical term or geographic name entry element |
Communicable diseases |
| General subdivision |
Diagnosis |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
46042 |
| Topical term or geographic name entry element |
DNA microarrays |
| General subdivision |
Diagnostic use |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |