000			nam a22 7a 4500
999			_c111129 _d111129
003			IE-CoIT
005			20180322113433.0
007			ta
008			180320s2017 ie ||||| |||| 00| 0|eng||
040			_aIE-CoIT
082	0	4	_aTHESES PRESS
100	1		_9123947 _aKennedy, Aideen _eauthor
245	1	2	_aA diagnostic and control programme for Johne's Disease on Irish dairy farms / _cAideen Kennedy.
264		1	_aCork : _bCork Institute of Technology, _c2017.
300			_a229 pages : _billustrations (chiefly color) ; _c30 cm
336			_atext _btxt _2rdacontent
337			_aunmediated _bn _2rdamedia
338			_avolume _bnc _2rdacarrier
490		0	_aPh. D - Biological Sciences
500			_aJohne's disease (JD) is an enteritis of ruminants with potential zoonotic implications. JD diagnosis ad control present difficulties give JDs prolonged subclinical nature. In order to improve JD control in Ireland, PhD aims included conducting a national survey documenting high-risk husbandry practices employed on Irish farms, thereby targeting areas for improved management. Furthermore as JD has been associated with on-farm economic losses internationally, this research programmed aimed to analyse production losses associated with testing JD ELISA positive on Irish dairy farms. A longitudinal study was also conducted to assess if successful JD control can be achieved using gold-standard protocols. Due to the prolonged nature of the disease, diagnosis of MAP is notoriously difficult. None of the available tests for MAP report perfect test sensitivity or specificity. A further complicating issue in Ireland, is the high level of bovine tuberculosis (bTB) testing that is conducted. Multiple investigations relating to bTB-testing and its relationship with MAP diagnostics were conducted. Across all investigations, 312 farms were recruited, samples were collected from 4500 cows, and 10000 test results were generated (ELISA, PCR, faecal culture). Irish farms engaged in high-risk management practices facilitating JD transmission, however, no significant associations were identified between JD ELISA positivity and production parameters. On-farm sero-prevalence was decreased by implementation of gold standard protocols. Eradication was not achieved, however, highlighting the long-term commitment required for effective JD control. Annual bTB testing was associated with an increased prevalence of JD ELISA positive results and indicates that sampling for the purposes of JD surveillance should be avoided for 71 days post- TB test administration. Antibody responses and interferon-y production were significantly increased post-b TB testing. As cell-mediated immunity is particularly important in the control of JD systemically, bTB testing may be contributing to the low levels of clinical JD in Ireland and warrants further investigation. This research programme has greatly contributed to our knowledge of JD in Ireland and improves understanding of practical measures necessary to interpret ELISA diagnostics and aid JD control - (Abstract)
500			_aThis Ph.D is a joint research project between Teagasc Moorepark and Cork Institute of Technology.
502			_aThesis _b(Ph. D.) - _cCork Institute of Technology, _d2017.
504			_aBibliography: (pages 203-229)
650		0	_993633 _aParatuberculosis _xDiagnosis _zIreland
650		0	_993633 _aParatuberculosis _xPrevention and control. _zIreland
650		0	_993631 _aMycobacterium avium paratuberculosis
650		0	_936972 _aEnzyme-linked immunosorbent assay
650		0	_936051 _aDairy farming _xManagement _zIreland
942			_2ddc