Techniques in molecular medicine / Friedhelm Hildebrandt and Peter Igarashi (eds.)
Contributor(s): Hildebrande, Friedhelm | Igarashi, Peter.
Material type: BookSeries: Springer lab manual.Publisher: Berlin : Springer, 1999Description: xii, 387 p. : ill. (some col.) ; 24 cm.ISBN: 3540571299 .Subject(s): Molecular biology | Molecular diagnosis | Medical geneticsDDC classification: 572.8078Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|---|
General Lending | MTU Bishopstown Library Lending | 572.8078 (Browse shelf(Opens below)) | 1 | Available | 00075587 |
Enhanced descriptions from Syndetics:
This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual
Includes bibliographical references and index.
Part I Molecular Biology Techniques: Structure and function of the human genome / Friedhelm Hildebrandt -- Enzymatic modification of DNA and RNA / Kai-Olaf Netzer -- Purification and nucleic acids from eukaryotic cells / Hans Gerd Nothwang and Friedhelm Hildebrandt -- Electrophoresis of DNA and RNA fragments / Feng Qian and Gregory G. Germino -- Single-strand conformation polymorphism (SSCP) analysis / Feng Qian and Gregory G. Germino -- Denaturing gradient gel electrophoresis / Kai-Olaf Netzer -- Introduction of pulsed-field gel electrophoresis (PFGE) / Feng Qian and Gregory G. Germino -- Pulsed-field gel electrophoresis: protocols / Gudrun A. Rappold, Karin Ried, Albrecht Klink, Ercole Rao and Birgit Weiss -- Radioactive labeling of DNA and RNA probes / Peter Igarashi -- Hybridization methods (southern and northern blotting) / Kai-Olaf Netzer -- Specimen preparation techniques for cytogenetic analysis by fluorescence in situ hybridization / Stefan Joos, Martin Bentz, Anton H. N. Hopman and Peter Lichter -- Chromosome analysis by fluorescence in situ hybridization / Martin Bentz, Stefan Joos and Peter Lichter -- Sequencing / Klaus Deichmann -- Polymerase chain reaction / Friedhelm Hildebrandt and Iva Singh-Sawhney -- Cloning vectors / Annette Deichmann and Klaus Deichmann -- Part II Strategies for gene isolation and characterization: Cloning strategies: an overview / Friedhelm Hildebrandt -- Geomic libraries / Luitz F. Onuchic and Gregory C. Germino -- cDNA libraries / Luitz F. Onuchic and Gregory G. Germino -- cDNA library screening / Johann Peter Hossle -- Expression cloning with xenopus oocytes / Daniel Markovich, Andreas Werner and Heini Murer -- Transgenic animals / Eckart Schott, Martin Paul and Detlev Ganten -- Expression in eukaryotic cells: gene transfer using retroviral vectors / Martin Marx -- Positional cloning and linkage analysis / Friedhelm Hildebrandt and Heymut Omran -- Part III Protein Methods: Protein Techniques / Robert F. Reilly.