Free radicals : a practical approach / edited by Neville A. Punchard and Frank J. Kelly.
Contributor(s): Punchard, Neville A | Kelly, Frank J.
Material type: BookSeries: Practical approach series: 168.Publisher: Oxford : IRL Press at Oxford University Press, 1996Description: xxv, 310 pages : illustrations ; 25 cm.Content type: text Media type: unmediated Carrier type: volumeISBN: 0199635609 ; 0199635595 .Subject(s): Free radicals (Chemistry) -- Analysis -- Laboratory manuals | Antioxidants -- Analysis -- Laboratory manualsDDC classification: 572.36Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|---|
General Lending | MTU Bishopstown Library Lending | 572.36 (Browse shelf(Opens below)) | 1 | Available | 00074310 |
Enhanced descriptions from Syndetics:
This volume brings together practical techniques for detecting and measuring free radicals - the reactive, short-lived molecules now known to play an important role in a large number of human diseases, including heart disease and cancer - and for studying their effects.
Includes bibliographical references and index.
Introduction / Neville A. Punchard and Frank J. Kelly -- Part I Free radical detection: physico-chemical methods: In vitro and in vivo detection of free radical metabolites with electron spin resonance / Ronald P. Mason -- Detection of free radical reaction products by high-field nuclear magnetic resonance spectroscopy / D. P. Naughton, E. Lynch, G. E. Hawkes, J. Hawkes, D. R. Blake and M. Grootveld -- Pulse radiolysis / J. Butler and E. J. Land -- Part II Free radical detection: biochemical methods: Intrinsic (low-level) chemiluminescence / Yury A. Vladimirov -- Visual assessment of oxidative stress by multifunctional digital microfluorography / Makoto Suematsu, Geert W. Schmid-Schonbein, Yuzuru Isimura and Masaharu Tsuchiya -- Salicylic acid and phenylalanie as probes to detect hydroxyl radicals / Harparkash Kaur and Barry Halliwell -- Part III Measurement of free radical products: Peroxides and other products / R. K. Brown and F. J. Kelly -- Quantitative analysis of 4-hydroxy-2-nonenal / Michael Kinter -- F2-Isoprostanes: prostaglandin-like products of lipid peroxidation / Jason D. Morrow and L. Jackson Roberts -- Determination of carbonyl groups in oxidized proteins / Emily Shacter, Joy A. Williams, Earl R. Stadtman and Rodney L. Levine -- Protein hydroperoxides, protein hydroxides and protein-bound DOPA / Roger T. Dean, Shanlin Fu, Steven Gieseg and Sharyn G. Armstrong -- Investigating the effects of oxygen free radicals on carbohydrates in biological systems / H. R. Griffiths and J. Lunec -- Measurement of products of free radical attack on nucleic acids / M. Lindsay Maidt and Robert A. Floyd -- Part IV Measurement of antioxidants: Glutathione / Mary E. Anderson -- Glutathione peroxidase: activity and steady-state level of mRNA / Daret K. St Clair and Ching K. Chow -- Superoxide dismutase / Sara Goldstein and Gidon Czapski -- Deuterated vitamin E: measurement in tissues and body fluids / Graham W. Burton and Malgorzata Daroszewska -- Selective and sensitive measurement of vitamin C, ubiquinol-10 and other low-molecular-weight antioxidants / Detlef Mohr and Roland Stocker -- Measurement of antioxidant gene expression / Colin D. Bingle.
Table of contents provided by Syndetics
- 1 Introduction
- 2 Direct and indirect measurements of free radicals by physico-chemical methods
- 2.1 In vitro and in vivo detection of free radical metabolites with electron spin resonance
- 2.2 The application of high-field nuclear magnetic resonance spectroscopy to the detiction of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs
- 2.3 Pulse radiolysis
- 3 Direct and indirect measurement of free radicals by other methods
- 3.1 Intrinsic (low-level) chemiluminescence
- 3.2 Visual assessment of oxidative stress by multifunctional digital microfluorography
- 3.3 Salicylic acid and phenylalanine as probes to detect hydroxyl radicals
- 4 Measurement of free radical products
- 4.1 Lipids (i): peroxides and other products
- 4.2 Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal
- 4.3 Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipik peroxidation
- 4.4 Proteins (i): determination of carbonyl groups in oxidized proteins
- 4.5 Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA
- 4.6 Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems
- 4.7 Nucleic acids: measurements of products of free radical attack on nucleic acids
- 5 Measurement of antioxidants
- 5.1 Glutahione
- 5.2 Glutathione peroxidase: activity and steady-state level of mRNA
- 5.3 Superoxide dismutase
- 5.4 Application of deuterium labelling and gas chromatographt-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids
- 5.5 Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants
- 5.6 Measurement of antioxidant gene expression