Protein function / edited by T.E. Creighton.

Contributor(s): Creighton, Thomas E, 1940-.

Material type: materialTypeLabel

BookSeries: Practical approach series ; 175.Publisher: Oxford ; New York : IRL Press at Oxford University Press, c1997Edition: 2nd ed.Description: xxi, 334 p. : ill. ; 25 cm. + pbk.ISBN: 0199636168 (v) (hbk); 019963615X (m) (pbk); 0199636176 (set : hbk).Subject(s): Proteins -- Analysis

| Protein binding -- Research -- Methodology | Proteins -- Structure-activity relationships | Proteins -- Affinity labeling

DDC classification: 547.75

Contents:

Identifying proteins for proteome studies: a two-dimensional gel electrophoresis approach / Jean-Charles Sanchez, Marc R. Wilkins, Ron D. Appel, Keith L. Williams and Denis F. Hochstrasser -- Expressing cloned genes in Eschericha coli / Steve Bass and Maria Yang -- Folding proteins / Rainer Rudolph, Gerald Bohm, Hauke Lilie and Rainer Jaenicke -- Ligand binding / Alexander Levitzki -- Ligand blotting / Anne K. Soutar and David P. Wade -- Affinity labelling / Roberta F. Colman -- Chemical cross-linking and protein function / Ronald Kluger -- Analysis of sequence-specific DNA-binding proteins / Daniela Rhodes and Louise Fairall -- Purification of sequence-specific DNA-binding proteins by affinity chromatography / Ken B. Kaplan and Peter K. Sorger -- Chemical modification / Taiji Imoto and Hidenori Yamada.

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode	Item holds
General Lending	MTU Bishopstown Library Lending	547.75 (Browse shelf(Opens below))	1	Available		00074715

Total holds: 0

Enhanced descriptions from Syndetics:

The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialisedequipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.

Companion v. to: Protein structure. 2nd ed. 1997.

Includes bibliographical references and index.

Table of contents provided by Syndetics

1 Identifying proteins by two-dimensional gel; electrophoresis
2 Expressing cloned genes in Escherichia coli
3 Folding proteins
4 Ligand binding
5 Ligand blotting
6 Affinity labelling
7 Chemical cross-linking and protein function
8 Analysis of sequence-specific DNA-binding proteins
9 Purification of sequence-specific DNA-binding proteins by affinity chromatography
10 Chemical modification

Reviews provided by Syndetics

CHOICE Review

This book and its companion volume Protein Structure (1989), also edited by Creighton, are part of "The Practical Approach Series." As with many of the other volumes in this series, this handbook is most useful for biochemical researchers. Each of the 11 chapters is written by experts in the field and the entire volume has been well edited. The topics include both the classical (e.g., minimizing protein inactivation, ligand binding affinities, cross-linking, chemical modifications) and newer aspects of protein function (e.g., site-directed mutagenesis, sequence-specific DNA binding proteins). Each chapter begins with an introduction and includes experimental and data analysis sections, conclusions, and references. The strength of this style is that both basic principles and detailed protocols are provided for each topic. Well written and effectively documented. For graduate students, technicians, and bench biochemists. D. Malamud University of Pennsylvania