Old, R. W. <br/><br/>
Principles of gene manipulation :  an introduction to genetic engineering / 
R. W. Old and S. B. Primrose. 
 - 4th ed. 
 - Oxford :  Blackwell Scientific Publications,  1989. 
 - viii, 438 p. :  col. ill. ;  25 cm. 
 - Studies in microbiology ;  v. 2 .
<br/><br/>Includes bibliographical references (p. 375-425) and index. 
<br/><br/>Section 1 Introduction to gene manipulation:  Basic techiques -- Cutting and joining DNA molecules -- Section 2 Cloning in E. coli:  Plasmids as cloning vehicles for use in E. coli -- Bacteriophage and cosmid vectors for E. coli -- Changing genes:  site-directed mutagenesis -- Analysing DNA sequences -- Cloning strategies, gene libraries and cDNA cloning -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Section 3 Cloning in organisms other than E. coli:  Cloning in bacteria other than E. coli -- Cloning in yeast and other microbial eukaryotes -- Gene transfer to plants -- Introducing genes into animal cells -- Transferring genes into animal oocytes, eggs and embryos -- Section 4 Applications of recombinant DNA technology:  Nucleic acid probes and their applications -- The impact of recombinant DNA technology:  the generation of novelty. 
<br/><br/><label>ISBN: </label>0632026081  
<br/><br/><label>LCCN: </label>89017540 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Genetic engineering.
<br/><br/><label>Dewey Class. No.: </label>575.1