Protein structure : a practical approach / edited by T. E. Creighton.
Contributor(s): Creighton, Thomas E
.
Material type: 
BookSeries: Practical approach series ; 174.Publisher: Oxford : Oxford University Press, 1997 (1998)Edition: 2nd ed.Description: xxiii, 383 p. : ill. ; 24 cm. + pbk.ISBN: 0199636184 ; 0019936192 ; 0199636176 ; 0199636206 .Subject(s): Proteins -- Analysis | Proteins -- Structure | Biochemistry -- TechniqueDDC classification: 572.633 | Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
| General Lending | MTU Bishopstown Library Lending | 572.633 (Browse shelf(Opens below)) | 1 | Available | 00074712 |
Enhanced descriptions from Syndetics:
New and updated, this second edition of Protein Structure presents electrophoretic, chromatographic, and spectrophotometric techniques as well as including new chapters on the relatively sophisticated methods of mass spectrometry and ultracentrifugation. The emphasis is not on the technical aspects of the instruments, but more on the practical aspects of sample preparation and the possibilities of experimental strategy and design. The companion volume to this book, Protein Function: A Practical Approach, details comparable simple techniques for characterizing protein function. Topics in this second volume include: protein molecular weight determination by SDS-PAGE; protein analysis by mass spectrometry; immunological detection of proteins of known sequence; identification of common post-translational modifications; peptide mapping; counting integral numbers of residues by chemical modification; disulfide bonds between cysteine residues; analysis of protein conformation by gel electrophoresis; hydrodynamic properties of proteins; determining the molar absorbance coefficient of a protein; optical spectroscopy to characterize protein conformation and conformational changes; measuring the conformational stability of a protein; imunochemical analysis of protein conformation; stabilization of protein structure by solvents. Protein Structure: A Practical Approach will prove invaluable to all researchers, from PhD students to experienced group leaders.
Companion v. to: Protein function. 2nd ed. 1997.
Includes bibliographical references and index.
Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis / Gregory S. Makowski and Melinda L. Ramsby -- Protein analysis by mass spectrometry / Ole Norregaard Jensen, Andrej Shevchenko and Matthias Mann -- Immunological detection of proteins of known sequence / Karl Heinz Scheidtmann, Silke Reinartz and Harald Schlebusch -- Identification of common post-translational modifications / Radha G. Krishna and Finn Wold -- Peptide mapping / Nick A. Morrice and Elizabeth A. Carrey -- Counting integral numbers of residues by chemical modification / Michelle Hollecker -- Disulfide bonds between cysteine residues / William R. Gray -- Analysis of protein conformation by gel electrophoresis / David P. Goldenberg -- Hydrodynamic properties of proteins / Stephen E. Harding -- How to determine the molar absorbance coefficient of a protein / C. Nick Pace and Franz X. Schmid -- Optical spectroscopy to characterize protein conformation and conformational changes / Franz X. Schmid -- Measuring the conformational stability of a protein / C. Nick Pace and J. Martin Scholtz -- Immunochemical analysis of protein conformation / Bertrand Friguet, Lisa Djavadi-Ohaniance and Michel E. Goldberg -- Stabilization of protein structure by solvents / Serge N. Timasheff and Tsutomu Arakawa.
Table of contents provided by Syndetics
- 1 Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis
- 2 Protein analysis by mass spectrometry
- 3 Immunological detection of proteins of known sequence
- 4 Identification of common post-translational modifications
- 5 Peptide mapping
- 6 Counting integral numbers of residues by chemical modification
- 7 Disulfide bonds between cysteine residues
- 8 Analysis of protein conformation by gel electrophoresis
- 9 Hydrodynamic properties of proteins
- 10 How to determine the molar absorbance coefficient of a protein
- 11 Optical spectroscopy to characterize protein confirmation and conformational changes
- 12 Measuring the conformational stability of a protein
- 13 Immunochemical analysis of protein conformation
- 14 Stabilization of protein structure by solvents