Mutation detection : a practical approach detection / edited by R.G.H. Cotton, E. Edkins and S. Forrest.
Contributor(s): Cotton, Richard G. H | Edkins, E. (Edward) | Forrest, S. (Sue).
Material type: BookSeries: Practical approach series: 188.Publisher: Oxford ; New York : IRL Press at Oxford University Press, 1998Description: xx, 242 p. : ill. ; 24 cm.ISBN: 0199636575 ; 0199636567 .Subject(s): Mutation (Biology) -- Laboratory manuals | Molecular genetics -- Laboratory manuals | Chromosome abnormalities -- Laboratory manualsDDC classification: 576.549Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|---|
General Lending | MTU Bishopstown Library Lending | 576.549 (Browse shelf(Opens below)) | 1 | Available | 00074719 |
Enhanced descriptions from Syndetics:
Although important in a great many fields, mutation detection is both time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
Includes bibliographical references and index.
Introduction / Sue Forrest -- Single-strand conformation polymorphism analysis / Kenshi Hayashi, Youji Kukita, Masakazu Inazuka and Tomoko Tahira -- Single-stranded conformation polymorphism and heteroduplex analysis / Bernard Gerrard and Michael Dean -- Comprehensive mutation detection with denaturing gradient gel electrophoresis / L. S. Lerman and Cherif Beldjord -- Cleavage using RNase to detect mutations / H. Nagase and Y. Nakamura -- Cleavage of mismatched bases using chemical reagents / Francesco Giannelli, Peter M. Green and Susan J. Ramus -- Mutation detection using T4 endonuclease VII / Rima Youil -- Detection of mutations by hybridization with sequence-specific oligonucleotide probes / Randall K. Saiki and Henry A. Erlich -- DNA detection and sequence distinction through oligonucleotide ligation / Ulf Landegren, Martina Samiotaki, Marek Kwiatkowski, Juri Parik, Mats Nilsson, Anette Hagberg and Gisela Barbany -- Detection of sequence variation using primer extension / Ann-Christine Syvanen -- Selective amplification of specific alleles / Cynthia D. K. Bottema and Steve S. Sommer -- The protein truncation test (PTT) / Rob B. van der Luijt, Riccardo Fodde and Johan T. den Dunnen -- Functional assay of the p53 tumour suppressor gene / Thierry Frebourg, Jean-Michel Flaman, Anne Estreicher and Richard Iggo -- Advances in direct DNA sequencing for mutation scanning / Joakim Lundeberg, Cecilia Williams, Asfhin Ahmadian and Mathias Uhlen.
Table of contents provided by Syndetics
- 1 Single strand conformation polymorphism analysis
- 2 Single-stranded conformation polymorphism and heteroduplex analysis
- 3 Comprehensive mutation detection with denaturing gradient gel electrophoresis
- 4 Cleavage using RNase to detect mutations
- 5 Cleavage of mismatched bases using chemical reagents
- 6 Mutation detection using T4 endonuclease VII
- 7 Detection of mutations by hybridization with sequence-specificoligonucleotide probes
- 8 DNA detection and sequence distinction through oligonucleotide ligation
- 9 Detection of sequence variation using primer extension
- 10 Selective amplification of specific alleles
- 11 The protein truncation text (PTT)
- 12 Functional assay of the p53 tumour suppressor gene
- 13 Advances in direct DNA sequencing for mutation scanning