Principles of gene manipulation : an introduction to genetic engineering / R. W. Old and S. B. Primrose.
By: Old, R. W.
Contributor(s): Primrose, S. B.
Material type: BookSeries: Studies in microbiology ; v. 2.Publisher: Oxford : Blackwell Scientific Publications, 1989Edition: 4th ed.Description: viii, 438 p. : col. ill. ; 25 cm.ISBN: 0632026081 .Subject(s): Genetic engineeringDDC classification: 575.1Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
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General Lending | MTU Bishopstown Library Lending | 575.1 (Browse shelf(Opens below)) | 1 | Available | 00043102 |
Includes bibliographical references (p. 375-425) and index.
Section 1 Introduction to gene manipulation: Basic techiques -- Cutting and joining DNA molecules -- Section 2 Cloning in E. coli: Plasmids as cloning vehicles for use in E. coli -- Bacteriophage and cosmid vectors for E. coli -- Changing genes: site-directed mutagenesis -- Analysing DNA sequences -- Cloning strategies, gene libraries and cDNA cloning -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Section 3 Cloning in organisms other than E. coli: Cloning in bacteria other than E. coli -- Cloning in yeast and other microbial eukaryotes -- Gene transfer to plants -- Introducing genes into animal cells -- Transferring genes into animal oocytes, eggs and embryos -- Section 4 Applications of recombinant DNA technology: Nucleic acid probes and their applications -- The impact of recombinant DNA technology: the generation of novelty.
Table of contents provided by Syndetics
- Introduction to Gene Manipulation
- Why do we clone genes?
- How do we clone genes?- the basic technique
- Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors
- Phage and cosmid vectors
- Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes
- Analyzing DNA nucleotide sequences
- Changing genes - macro- and micro-modifications
- The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics
- Cloning in Organisms other than E. coli
- Cloning in bacteria other than E. coli
- Cloning in yeast and other microbial eukaryotes
- Gene transfer to plants
- Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty