Principles of gene manipulation : an introduction to genetic engineering / R. W. Old and S. B. Primrose.
By: Old, R. W
.
Contributor(s): Primrose, S. B
.
Material type: ![materialTypeLabel](/opac-tmpl/lib/famfamfam/BK.png)
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Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
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General Lending | MTU Bishopstown Library Lending | 575.10724 (Browse shelf(Opens below)) | 1 | Available | 00009793 |
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Enhanced descriptions from Syndetics:
As in past editions, the basic philosophy of this extremely popular text is to present the principles and techniques of gene manipulation in sufficient detail to enable the non-specialist to understand them. However, in the three years since the previous edition, molecular biology has seen a dramatic increase in available techniques and applications. Every chapter in this new edition has been extensively revised or rewritten to take these developments into account - including a vastly expanded section on the polymerase chain reaction and its manifold applications, a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics, and extended coverage of gene transfer techniques.
Includes bibliographical references (p. 412-464) and index.
Gene manipulation: an all-embracing technique -- Basic techniques -- Cutting and joining DNA molecules -- Plasmids as cloning vehicles for use in E. coli --- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies, gene libraries and cDNA cloning -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Analysing DNA sequences -- The polymerase chain reaction -- Changing genes: site-directed mutagenesis -- Cloning in bacteria other than E. coli -- Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- Gene transfer to plants -- Introducing genes into animal cells -- Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- The impact of recombinant DNA technology: the generation of novelty.
Table of contents provided by Syndetics
- Introduction to Gene Manipulation
- Why do we clone genes?
- How do we clone genes?- the basic technique
- Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors
- Phage and cosmid vectors
- Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes
- Analyzing DNA nucleotide sequences
- Changing genes - macro- and micro-modifications
- The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics
- Cloning in Organisms other than E. coli
- Cloning in bacteria other than E. coli
- Cloning in yeast and other microbial eukaryotes
- Gene transfer to plants
- Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty