Expression genetics : differential display / edited by Arthur B. Pardee and Michael McClelland.
Contributor(s): Pardee, Arthur B. (Arthur Beck)
| McClelland, Michael.
Material type: 
BookSeries: BioTechniques update series.Publisher: Natick, MA : BioTechniques Books, 1999Description: xxv, 488 p. : ill. ; 24 cm.ISBN: 1881299317.Subject(s): Gene expression -- Research. -- Methodology | Polymerase chain reaction | DNA fingerprinting | Messenger RNADDC classification: 572.865 | Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
| General Lending | MTU Bishopstown Library Lending | 572.865 (Browse shelf(Opens below)) | 1 | Available | 00082756 | ||
| General Lending | MTU Bishopstown Library Lending | 572.865 (Browse shelf(Opens below)) | 1 | Available | 00082755 |
Enhanced descriptions from Syndetics:
Reprints 60 articles, many with updates by the authors, from the journal about a fingerprinting technology that facilitates identifying which mRNA are involved in the molecular control of many normal or pathological processes. Paralleling the steps of the process, they cover preparing samples, first strand DNA synthesis, polymerase chain reaction, electrophoresis, isolating PCR products, confirming the products, and cloning. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Includes bibliographical references and index.
Section I Sample preparation: A. RNA extraction -- B. DNA removal -- Section II First strand DNA synthesis: A. Primers -- B. Reducing false positives -- C. Reverse transcription conditions -- Section III Polymerase chain reaction -- Section IV Electrophoresis -- Section V Isolation of PCR products: A. Labeling methods -- B. Collection of bands -- C. Reamplifying bands -- Section VI Confirmation of products -- Section VII Cloning.