Biotechnology explorations : applying the fundamentals / Judith A. Scheppler, Patricia E. Cassin and Rosa M. Gambier.
By: Scheppler, Judith A
.
Contributor(s): Cassin, Patricia E | Gambier, Rosa M.
Material type: 
BookPublisher: Washington, DC : ASM Press, 2000Description: ix, 277 p. : ill. (some col.) ; 28 cm.ISBN: 1555811787.Subject(s): Biotechnology -- Study and teaching | Biotechnology -- Laboratory manualsDDC classification: 660.6078 | Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
| General Lending | MTU Bishopstown Library Lending | 660.6078 (Browse shelf(Opens below)) | 1 | Available | 00086345 |
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Enhanced descriptions from Syndetics:
A broad collection of college-level experiments that provides students with a hands-on understanding of biotechnology and molecular biology, including applications and practical uses.* Includes suggested reading, laboratory language, and analysis questions that help direct student thinking. * Sidebar comments offer special hints and detailed information to ensure success but keep the protocols themselves easy to follow. * Comprehensive laboratory safety guidelines are also included. The experiments, organized into fundamental laboratory activities and applications of these protocols, can be adapted to fit the time frame of a one- or two-semester laboratory course.
Includes bibliographical references.
The fundamentals -- Section I: Getting started -- Section II: The techniques -- Applying the fundamentals -- Section III: DNA Cloning -- Section IV: Southern blotting -- Section V: Protein studies -- Section VI: Gene transfer -- Section VII: Techniques for studying evolution and systematics -- Section VIII: Internet- and computer-assisted gene analysis -- Section IX: Advanced techniques.
Table of contents provided by Syndetics
- Preface (p. vii)
- Acknowledgments (p. ix)
- Laboratory Safety (p. 1)
- The Fundamentals (p. 9)
- Section I Getting Started (p. 11)
- Exercise 1 Documentation of Laboratory Experiments (p. 11)
- Exercise 2 Use of Equipment (p. 15)
- Exercise 3 Preparation of Solutions (p. 23)
- Exercise 4 Preparation and Maintenance of Microbial Cultures (p. 31)
- Exercise 5 Isolation of DNA by Spooling (p. 41)
- Exercise 6 Investigating the Structure and Bond Strength of DNA (p. 45)
- Section II The Techniques (p. 49)
- Exercise 7 Restriction Enzyme Analysis of Lambda DNA (p. 49)
- Exercise 8 Miniprep of Plasmid DNA (p. 59)
- Exercise 9 Bacterial Transformation (p. 67)
- Exercise 10 Amplification of Human DNA by the Polymerase Chain Reaction (p. 75)
- Exercise 11 Quantitation of DNA by Spectrophotometry (p. 85)
- Exercise 12 Quantitation of DNA by Gel Electrophoresis (p. 91)
- Applying the Fundamentals (p. 97)
- Section III DNA Cloning (p. 99)
- Exercise 13 DNA Ligation and Cloning (p. 99)
- Exercise 14 DNA Cloning by Polymerase Chain Reaction (p. 113)
- Exercise 15 DNA Cloning for Protein Expression (p. 127)
- Section IV Southern Blotting (p. 141)
- Exercise 16 Restriction Fragment Length Polymorphism Analysis of Human DNA (p. 141)
- Section V Protein Studies (p. 157)
- Exercise 17 Quantitation of Protein (p. 157)
- Exercise 18 Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Coomassie Blue Staining of Protein (p. 163)
- Exercise 19 Western Blotting and Immunodetection (p. 171)
- Section VI Gene Transfer (p. 187)
- Exercise 20 Transformation of Yeasts (p. 187)
- Exercise 21 Transformation of Plants (p. 195)
- Section VII Techniques for Studying Evolution and Systematics (p. 203)
- Exercise 22 Isolation of Genomic DNA (p. 203)
- Exercise 23 Comparison of DNAs from the Five Kingdoms (p. 215)
- Exercise 24 Evolution and Diversity through Protein Homologies (p. 219)
- Section VIII Internet- and Computer-Assisted Gene Analysis (p. 225)
- Exercise 25 Internet Gene Bank Search (p. 225)
- Exercise 26 Computer-Assisted Sequence Analysis (p. 233)
- Exercise 27 Oligonucleotide Design by Computer (p. 241)
- Section IX Advanced Techniques (p. 251)
- Exercise 28 DNA Sequencing (p. 251)
- Exercise 29 DNA Library Construction (p. 259)
- Appendix (p. 271)