MTU Cork Library Catalogue

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Principles of gene manipulation : an introduction to genetic engineering / R. W. Old and S. B. Primrose.

By: Old, R. W.
Contributor(s): Primrose, S. B.
Material type: materialTypeLabelBookSeries: Studies in microbiology ; v.2.Publisher: Oxford : Blackwell Scientific, 1985Edition: 3rd ed.Description: xiii, 409 p. : ill. (some col.) ; 24 cm.ISBN: 0632013184.Subject(s): Genetic engineeringDDC classification: 575.1
Contents:
Section 1 Introduction to gene manipulation: Basic techniques -- Cutting and joining DNA molecules -- Section 2 Cloning in E. coli: Plasmids as cloning vehicles for use in E. coli -- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies and gene libraries -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Section 3 Cloning in organisms other than E. coli: Cloning in bacteria other than E. coli -- Cloning in yeast and other microbial eukaryotes -- Cloning in plant cells -- Cloning in mammalian cells in culture -- Microinjecting genes into animal oocytes, eggs and embryos -- Section 4 Applications of recombinant DNA technology: Applications of recombinant DNA technology in biology and medicine -- Industrial applications of gene manipulation.
Holdings
Item type Current library Call number Copy number Status Date due Barcode Item holds
General Lending MTU Bishopstown Library Lending 575.1 (Browse shelf(Opens below)) 1 Available 00043652
Total holds: 0

Bibliography: p. 349-390. - Includes index.

Section 1 Introduction to gene manipulation: Basic techniques -- Cutting and joining DNA molecules -- Section 2 Cloning in E. coli: Plasmids as cloning vehicles for use in E. coli -- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies and gene libraries -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Section 3 Cloning in organisms other than E. coli: Cloning in bacteria other than E. coli -- Cloning in yeast and other microbial eukaryotes -- Cloning in plant cells -- Cloning in mammalian cells in culture -- Microinjecting genes into animal oocytes, eggs and embryos -- Section 4 Applications of recombinant DNA technology: Applications of recombinant DNA technology in biology and medicine -- Industrial applications of gene manipulation.

Table of contents provided by Syndetics

  • Introduction to Gene Manipulation
  • Why do we clone genes?
  • How do we clone genes?- the basic technique
  • Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors
  • Phage and cosmid vectors
  • Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes
  • Analyzing DNA nucleotide sequences
  • Changing genes - macro- and micro-modifications
  • The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics
  • Cloning in Organisms other than E. coli
  • Cloning in bacteria other than E. coli
  • Cloning in yeast and other microbial eukaryotes
  • Gene transfer to plants
  • Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty

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