Principles of gene manipulation : an introduction to genetic engineering / R. W. Old and S. B. Primrose.
By: Old, R. W
.
Contributor(s): Primrose, S. B
.
Material type: ![materialTypeLabel](/opac-tmpl/lib/famfamfam/BK.png)
![](/opac-tmpl/bootstrap/images/filefind.png)
Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|---|
General Lending | MTU Bishopstown Library Lending | 575.1 (Browse shelf(Opens below)) | 1 | Available | 00043652 |
Bibliography: p. 349-390. - Includes index.
Section 1 Introduction to gene manipulation: Basic techniques -- Cutting and joining DNA molecules -- Section 2 Cloning in E. coli: Plasmids as cloning vehicles for use in E. coli -- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies and gene libraries -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Section 3 Cloning in organisms other than E. coli: Cloning in bacteria other than E. coli -- Cloning in yeast and other microbial eukaryotes -- Cloning in plant cells -- Cloning in mammalian cells in culture -- Microinjecting genes into animal oocytes, eggs and embryos -- Section 4 Applications of recombinant DNA technology: Applications of recombinant DNA technology in biology and medicine -- Industrial applications of gene manipulation.
Table of contents provided by Syndetics
- Introduction to Gene Manipulation
- Why do we clone genes?
- How do we clone genes?- the basic technique
- Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors
- Phage and cosmid vectors
- Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes
- Analyzing DNA nucleotide sequences
- Changing genes - macro- and micro-modifications
- The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics
- Cloning in Organisms other than E. coli
- Cloning in bacteria other than E. coli
- Cloning in yeast and other microbial eukaryotes
- Gene transfer to plants
- Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty