Practice and theory of enzyme immunoassays / P. Tijssen.
By: Tijssen, P [author. ]
.
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Enhanced descriptions from Syndetics:
Paperback. Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.
Includes bibliographical references and index.
Introduction -- Outline of the strategies for enzyme immunoassays -- Non-immunologic molecular recognition systems used in immunoassays -- The nature of immunogens, antigens and haptens -- Production of antibodies -- The nature and structure of antibodies -- Purification of immunoglobulins and preparation of Fab fragments -- Kinetics and nature of antibody-antigen interactions -- The nature of enzyme activity in immunoassays -- Properties and preparation of enzymes used in enzyme immunoassays -- Preparation of enzyme-antibody or other enzyme-macromolecule conjugates -- Conjugation of haptens -- The immobilization of immunoreactants on solid phases -- Quantitative enzyme immunoassay techniques -- Processing of data and reporting of results of enzyme immunoassays -- Localization and identification of electrophoretically separated antigens after their transfer from gel to membrane -- Enzyme immunohistochemistry (EIH) in light and electron microscopy -- Potential toxic hazards associated with enzyme immunoassays.