MTU Cork Library Catalogue

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Practice and theory of enzyme immunoassays / P. Tijssen.

By: Tijssen, P [author. ].
Material type: materialTypeLabelBookSeries: Laboratory techniques in biochemistry and molecular biology: 15.Publisher: Amsterdam ; New York : New York : Elsevier ; Sole distributors for the USA and Canada, Elsevier Science Pub. Co., [1985]Copyright date: ©1985Description: xxvi, 549 pages : illustrations ; 21 cm.Content type: text Media type: unmediated Carrier type: volumeISBN: 0444806334 (paperback); 0444806342 (paperback).Subject(s): Immunoenzyme techniqueDDC classification: 616.0756
Contents:
Introduction -- Outline of the strategies for enzyme immunoassays -- Non-immunologic molecular recognition systems used in immunoassays -- The nature of immunogens, antigens and haptens -- Production of antibodies -- The nature and structure of antibodies -- Purification of immunoglobulins and preparation of Fab fragments -- Kinetics and nature of antibody-antigen interactions -- The nature of enzyme activity in immunoassays -- Properties and preparation of enzymes used in enzyme immunoassays -- Preparation of enzyme-antibody or other enzyme-macromolecule conjugates -- Conjugation of haptens -- The immobilization of immunoreactants on solid phases -- Quantitative enzyme immunoassay techniques -- Processing of data and reporting of results of enzyme immunoassays -- Localization and identification of electrophoretically separated antigens after their transfer from gel to membrane -- Enzyme immunohistochemistry (EIH) in light and electron microscopy -- Potential toxic hazards associated with enzyme immunoassays.
Holdings
Item type Current library Call number Copy number Status Date due Barcode Item holds
General Lending MTU Bishopstown Library Store Item 616.0756 (Browse shelf(Opens below)) 1 Available 00043489
Total holds: 0

Enhanced descriptions from Syndetics:

Paperback. Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.

Includes bibliographical references and index.

Introduction -- Outline of the strategies for enzyme immunoassays -- Non-immunologic molecular recognition systems used in immunoassays -- The nature of immunogens, antigens and haptens -- Production of antibodies -- The nature and structure of antibodies -- Purification of immunoglobulins and preparation of Fab fragments -- Kinetics and nature of antibody-antigen interactions -- The nature of enzyme activity in immunoassays -- Properties and preparation of enzymes used in enzyme immunoassays -- Preparation of enzyme-antibody or other enzyme-macromolecule conjugates -- Conjugation of haptens -- The immobilization of immunoreactants on solid phases -- Quantitative enzyme immunoassay techniques -- Processing of data and reporting of results of enzyme immunoassays -- Localization and identification of electrophoretically separated antigens after their transfer from gel to membrane -- Enzyme immunohistochemistry (EIH) in light and electron microscopy -- Potential toxic hazards associated with enzyme immunoassays.

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