Biotechnology : a laboratory course / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo.
By: Becker, Jeffrey M
.
Contributor(s): Caldwell, Guy A | Zachgo, Eve Ann.
Material type: 
BookPublisher: San Diego : Academic Press, c1990Description: xviii, 232 p. : ill. ; 24 cm.ISBN: 0120845601.Subject(s): Biotechnology -- Laboratory manuals | Biology -- Laboratory manualsDDC classification: 660.6 | Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
| General Lending | MTU Bishopstown Library Lending | 660.6 (Browse shelf(Opens below)) | 1 | Available | 00041221 |
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Enhanced descriptions from Syndetics:
A laboratory manual for an introductory course in biotechnology. A series of experiments integrate basic molecular biology and protein biochemistry using the bacterium Eschericha coli and the yeast Saccharomyces cerevisiae. Plastic comb binding. Annotation(c) 2003 Book News, Inc., Portland, OR (booknews.com)
Includes bibliographical references and index.
Introductory notes: Record keeping and safety rules -- Exercise 1: Measurement of pH -- Exercise 2: Use of micropipettors and spectrophotometers -- Exercise 3: Aseptic technique: Transferring a culture -- Exercise 4: Establishing a pure culture: The streak plate -- Exercise 5: Preparation of culture media -- Exercise 6: The growth curve -- Exercise 7: Isolation of plasmid DNA from Escherichia coli: The mini-prep -- Exercise 8: Purification, concentration and quantitation of DNA -- Exercise 9: Isolation of plasmid DNA: The maxi-prep -- Exercise 10: Restriction digestion and agarose gel electrophoresis -- Exercise 11: Southern transfer -- Exercise 12: Preparation, purification and hybridization of probe -- Exercise 13: Transformation of Saccharomyces cerevisiae -- Exercise 14: Transformation of Excherichia coli by plasmid DNA -- Exercise 15: Protein assays -- Exercise 16: B-Galactosidase assay -- Exercise 17: Determination of B-Galactosidase in permeabilized yeast cells -- Exercise 18: Assay of B-Galactosidase in cell extracts -- Exercise 19: B-Galactosidase purification.