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HPLC and CE [electronic book] : principles and practice / Andrea Weston and Phyllis R. Brown.

By: Weston, Andrea, 1965- [author].
Contributor(s): Brown, Phyllis R [author].
Material type: materialTypeLabelBookPublisher: San Diego : Academic Press, [1997]Copyright date: ©1997Description: online resource (xiv, 280 pages) : illustrations.Content type: text Media type: computer Carrier type: online resourceISBN: 9780121366407 (hardback); 9780080534169 (e-book).Other title: High performance liquid chromatography and capillary electrophoresis : principles and practices.Subject(s): High performance liquid chromatography | Capillary electrophoresisDDC classification: 543.0871 Online resources: E-book Summary: HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Key Features * Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques * Applicable to a wide variety of separation problems * Covers basic concepts governing any separation as well as instrumentation and how to use it * Helps the user to obtain optimal resolution in minimal time * Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation * Includes information on upcoming trends such as miniaturization * Major concepts in each chapter are organized to allow access to information easily and quickly * Contains practical bibliography for accessing the literature.

Enhanced descriptions from Syndetics:

Providing a source of information on the powerful separation techniques high performance liquid chromatography and capillary electrophoresis, this text presents the fundamental theory, instrumentation, modes of operation, and optimization of these separations. Also provided is coverage of troubleshooting techniques, improvement of resolution, data manipulation, selectivity, and sensitivity. Information is also included on special procedures such as chiral separations, affinity, chromatography, and sample preparation and current trends such as miniaturization.

Includes bibliographical references and index.

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Key Features * Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques * Applicable to a wide variety of separation problems * Covers basic concepts governing any separation as well as instrumentation and how to use it * Helps the user to obtain optimal resolution in minimal time * Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation * Includes information on upcoming trends such as miniaturization * Major concepts in each chapter are organized to allow access to information easily and quickly * Contains practical bibliography for accessing the literature.

Electronic reproduction.: ProQuest LibCentral. Mode of access: World Wide Web.

Table of contents provided by Syndetics

  • High Performance Liquid Chromatography, Introduction
  • Classification of Liquid Chromatographic Methods
  • Basic Concepts
  • Summary of Major Concepts
  • References
  • Separations in High Performance Liquid Chromatography, Introduction
  • Normal Phase Chromatography
  • Reversed Phase Chromatography
  • Hydrophobic Interaction
  • Ion Exchange Chromatography
  • Size Exclusion Chromatography
  • Affinity Chromatography
  • Chiral Separations
  • Summary of Major Concepts
  • References
  • Instrumentation for High Performance Liquid Chromatography, Introduction
  • Solvent Delivery Systems
  • Solvent Degassing
  • Isocratic Versus Gradient Pumping Systems
  • Sample Introduction
  • Columns
  • Detectors
  • Sample Preparation
  • Troubleshooting
  • Summary of Major Concepts
  • References
  • Capillary Electrophoresis, Introduction
  • Classification of Electrophoretic Modes
  • Basic Concepts
  • Summary of Major Concepts
  • Separations in Capillary Electrophoresis, Introduction
  • Capillary Zone Electrophoresis
  • Micellar Electrokinetic Capillary Chromatography
  • Capillary Gel Electrophoresis
  • Capillary Electromatography
  • Chiral Capillary Electrophoresis
  • Capillary Isoelectric Focusing
  • Capillary Isotachophoresis
  • Summary of Major Concepts
  • References
  • Instrumentation for Capillary Electrophoresis, Introduction
  • High Voltage Power Supply
  • Sample Injection
  • Capillaries
  • Detection
  • Fraction Collection
  • Sample Preparation
  • Troubleshooting
  • Summary of Major Concepts
  • References
  • Data Manipulation, Introduction
  • Identification of Peaks
  • Quantification
  • Summary of Major Concepts
  • References
  • Miniaturization, Introduction
  • Classification
  • Applications of Miniaturized LC
  • Separations on a Chip
  • Summary of Major Concepts
  • References

Reviews provided by Syndetics

CHOICE Review

This monograph outlines the basic concepts, the chemistry, and the instrumentation of high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). In addition, these two methods are compared in order to assist the practitioner in solving actual chemical separation problems. The first three chapters provide a clear, though rather brief, overview of the theory and practice of HPLC. Included in this section are such topics as normal and reverse phase separations, as well as ion exchange, size exclusion and affinity chromatography, and chiral separations. The chapter on HPLC instrumentation is particularly useful because of the many clear figures and the valuable troubleshooting guide. Chapters 4 through 6 present the theory and instrumentation of a variety of CE techniques such as capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC), capillary gel electrophoresis (CGE), capillary electrochromatography (CEC), capillary isoelectric focusing (CIEF), and chiral capillary electrophoresis. The remaining two chapters discuss the topics of data manipulation and miniaturization. Extensive chapter references; useful index. A well written "must" for all users of HPLC and/or CE and for nonpractitioners who wish a basic understanding of these important separation techniques. Upper-division undergraduates through professionals. H. T. McKone Saint Joseph College

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