MARC details
| 000 -LEADER |
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| fixed length control field |
02941nam a22002657a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
IE-CoIT |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20181019155242.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
181016s2018 ie ||||| |||| 00| 0|eng|| |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
IE-CoIT |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
THESES PRESS |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
125484 |
| Personal name |
Ajuebor, Jude |
| Relator term |
author |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Phage therapy and development of delivery systems for Gram-positive phage endolysins / |
| Statement of responsibility, etc. |
Jude Ajuebor. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Cork : |
| Name of producer, publisher, distributor, manufacturer |
Cork Institute of Technology, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2018. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
ix, 221 pages : |
| Other physical details |
color illustrations, tables ; |
| Dimensions |
30 cm |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
unmediated |
| Media type code |
n |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Carrier type code |
nc |
| Source |
rdacarrier |
| 490 0# - SERIES STATEMENT |
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| Series statement |
Ph.D - Biological sciences |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis |
| Degree type |
|
| Name of granting institution |
Cork Institute of Technology, |
| Year degree granted |
2018. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references. |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
"This thesis focused on Gram-positive phages and their endolysins. Here, two similar kay-like staphylococcal phages B1 (vB_SauM_B1) and JA1 (vB_SauM-JA1) were isolated from a commercial therapeutic phage mix. Their host range was established on the Irish National MRSA bank, which included twenty one sequence types in addition relevant control strains. Based on this, distinct phages were identified and subjected to genome sequencing. The sequences were compared with the sequence of phage K (vB_SauM_K), which was also determined in this work. All three phages had a genome size of at least 139 kb, although some key differences were identified between each. The new phages B1 and JA1 possessed double stranded DNA and generally had a broader host range than phage K. A comparative genomic analysis on the phage genomes identified several (open reading frames) ORFs that were absent in the genome of phage K but present in genomes of phages B1 and JA1. One of the cloned genes from phage K was shown to encode a protein for the receptor-binding-protein and this protein was demonstrated to slightly inhibit phage adsorption. The other cloned gene encoded the phage endolysin and this peptidoglycan hydrolase were identical across all three phages and thus, the CHAPk endolysin of phage K was chosen to demonstrate the application of the endolysin for the control of staphylococci in milk. A two-log reduction in staphylococcal numbers in milk was observed. When the endolysin was introduced into a lactococcal secretion system using the pNZ8048 vector, detectable secretion was successfully demonstrated. Simultaneously, a Clostridium difficile phage endolysin, an amidase, was also cloned into the same secretion system with successful secretion also being demonstrated. In addition, this latter endolysin was also secreted from a recombinant E. coli strain, suggesting potential applications for delivery of the endolysin to the intestine from a hypothetical probiotic e. coli strain". Abstract |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
45340 |
| Topical term or geographic name entry element |
Gram-positive bacteria |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
52169 |
| Topical term or geographic name entry element |
Bacteriophages |
| General subdivision |
Therapeutic use |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |