MTU Cork Library Catalogue

Inhibition of Listeria monocytogenes by human gut bacteria / (Record no. 112499)

MARC details
000 -LEADER
fixed length control field 04362nam a22003137a 4500
003 - CONTROL NUMBER IDENTIFIER
control field IE-CoIT
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20181017150047.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 181016s2018 ie ||||| |||| 00| 0|eng||
040 ## - CATALOGING SOURCE
Original cataloging agency IE-CoIT
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number THESES PRESS
100 1# - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 125473
Personal name Oleksy, Patrycja
Relator term author
245 10 - TITLE STATEMENT
Title Inhibition of Listeria monocytogenes by human gut bacteria /
Statement of responsibility, etc. Patrycja Oleksy
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Cork :
Name of producer, publisher, distributor, manufacturer Cork Institute of Technology,
Date of production, publication, distribution, manufacture, or copyright notice 2018.
300 ## - PHYSICAL DESCRIPTION
Extent v, 141 pages :
Other physical details illustrations (some color), tables ;
Dimensions 30 cm
336 ## - CONTENT TYPE
Content type term text
Content type code txt
Source rdacontent
337 ## - MEDIA TYPE
Media type term unmediated
Media type code n
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term volume
Carrier type code nc
Source rdacarrier
490 0# - SERIES STATEMENT
Series statement M.Sc. - Biological Sciences
502 ## - DISSERTATION NOTE
Dissertation note Thesis
Degree type
Name of granting institution Cork Institute of Technology,
Year degree granted 2018.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Bibliography: (pages 108-138)
520 3# - SUMMARY, ETC.
Summary, etc. "Listeria monocytogenes is a foodborne pathogen, which continues to be problematic to the food industry due to its ability to survive within food products and persist within food processing environments. The growing trend towards natural food preservatives and antimicrobial agents increases the need for the development of novel, natural anti-Listeria agents. Evidence presented in the literature suggests that the human gut microbiota is a reservoir of novel antimicrobial agents. It was therefore hypothesized that novel anti-Listeria agents are produced by human gut-derived bacteria. The object of this study was to isolate and characterize gut bacteria with anti-Listeria monocytogenes activity. A total of 23 fecal banks of human origin were screened for antagonistic activity against L.monocytogenes 10403S. The results of the initial screening have shown that 1,569 gut-derived isolates have demonstrated various levels of anti-Listeria activity. Following an extensive screen 59 gut-derived isolates were initially shortlisted and were identified mostly as Enterococcus spp. By a combination of MALDI-TOF MS analysis and 16S rRNA gene sequencing. The data collected allowed a further shortlisting of strains from 59 to 16 based on differences observed in the colony morphology, the size and appearance of the zones of inhibition observed in the deferred antagonism assays and well diffusion assays as well as spectrum of activity of each isolate against a umber of indicator strains. The antimicrobials produced by 11 out of 16 shortlisted gut derived isolates were identified as peptides using a proteinase K assay. The anti-Listeria activity of the shortlisted isolates were examined in a model broth co-culture experiment. Based on the results obtained the antimicrobials produced by two Enterococcus faecium isolates were selected for further investigation. The activity of the antimicrobials were investigated in dairy food homogenate models and stainless steel biofilm assays. The findings of the food trials have shown a reduction of Listeria numbers by ~0.6 log CRFU/mL in natural yogurt treated with cell free supernatent (CFS) and a reduction of ~1.5 log CFU/mL observed in cheddar cheese treated with whole cell extract (WCE) following a 3 hr incubation. The anti-biofilm assays demonstrated a reduction in biofilm formation of stainless steel by 70% when treated with CFS and 92.42% when treated with (WCE). The experiments presented in this thesis confirm that bacteria from the human gut produce anti-Listeria compounds which can control or reduce the numbers of Listeria in model co-cultures and in food homogenates. The antimicrobials isolated in this study have also shown the ability to reduce Listeria biofilm formation. Future efforts should focus on identifying the antimicrobials produced by all of the shortlisted strains (through a combination of genome sequencing and peptide purifications). It is hoped that any novel antimicrobial that is discovered have an application in the food industry to reduce the significant threat posed by L. monocytogenes. These antimicrobials could be used as a natural preservative in food products and packaging or incorporated into a spray to control biofilm in the food processing environment". Abstract
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 44779
Topical term or geographic name entry element Listeria monocytogenes
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 44778
Topical term or geographic name entry element Listeria
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 44765
Topical term or geographic name entry element Foodborne diseases
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 37454
Topical term or geographic name entry element Food industry and trade
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 39915
Topical term or geographic name entry element Microbial metabolism
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 37714
Topical term or geographic name entry element Gastrointestinal system
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Cost, normal purchase price Total Checkouts Full call number Barcode Date last seen Cost, replacement price Price effective from Koha item type
    Dewey Decimal Classification   Reference MTU Bishopstown Library MTU Bishopstown Library Thesis 16/10/2018 25.00   THESES PRESS 00181363 16/10/2018 25.00 31/03/2021 Reference

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