MARC details
| 000 -LEADER |
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| fixed length control field |
nam a22 7a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
IE-CoIT |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20180320145733.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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| fixed length control field |
ta |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
180316s2017 ie ||||| |||| 00| 0|eng|| |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
IE-CoIT |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
THESES PRESS |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
123926 |
| Personal name |
Bane, Vaishali |
| Relator term |
author |
| 245 ## - TITLE STATEMENT |
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| Title |
Application of LC-MS technology for the detection of emerging marine toxins from the European region / |
| Statement of responsibility, etc. |
Vaishali Bane. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Cork : |
| Name of producer, publisher, distributor, manufacturer |
Cork Institute of Technology, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2017. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
460 pages : |
| Other physical details |
illustrations (some color) ; |
| Dimensions |
30 cm |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
unmediated |
| Media type code |
n |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
volume |
| Carrier type code |
nc |
| Source |
rdacarrier |
| 490 #0 - SERIES STATEMENT |
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| Series statement |
Ph.D. - Physical Sciences |
| 500 ## - GENERAL NOTE |
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| General note |
The seafood industry is economically one of the biggest food industries in the world. Seafood is a rich source of high quality protein (=16% of animal protein consumed by world's population). Therefore, the contamination of seafood with marine toxins is a major concern for businesses worldwide and for consumer public health. Since 2004, the European Union monitors and legislates for the most common types of biotoxins found in European waters; diarrhetic shellfish poisoning (DSP), amnesic shellfish poisoning (ASP) and paralytic shellfish poisoning (PSP). However, over the past decade new (emerging) classes of marine toxins has started appearing in European water and shellfish produce. The emerging class of toxins includes tetrodotoxin (TTX), palytoxin (PITX) and cyclic imine (CI) toxins. The emergence of these toxins in European waters has been attributed to global warming, lessepsian migration or from ballast water discharge.These emerging toxins are not monitored in Europe from the prospective of seafood safety. Also, the regulatory limits are not established for these toxins. Mouse assay was the traditional reference method for monitoring native toxins in Europe. However, since Jan 2015, the EU Commission Regulations No. 15/2011 of 10 Jan 2011, states that Liquid Chromatography - Mass Spectrometry (LC-MS) is the preferred reference method for marine toxin detection. The main reason for avoiding use of the mouse assay is ethical concerns regarding the use of animals and its lack of robustness, toxin group selectivity and sensitivity. LC-MS analysis of marine toxins offers selective identification and sensitive, yet robust, quantification abilities. The main objective of this Ph.D. is the development of a robust LC-MS methods for detecton of tetrodotoxin and cyclic imine toxins. Quantitative LC-MS methods were developed and validated for tetrodotoxin, cyclic imine and other common lipophilic toxin groups on a triple quadrupole (QqQ) and Orbittrap Fourier Transform Mass Spectrometers (FT-MS). The major challenge in the development of multi-toxin LC-MS methods is the lack of certified reference materials for minor analogues of toxins. This problem was circumvented with the use of Orbitrap FT-MS. The analogues of toxins were detected using untargeted full scan and data dependent scanning modes of Orbitrap FT-MS. Thus, the methods presented in this thesis have the capability to screen untargeted toxin compounds such as structural isomers or the metabolites of the toxins. The high resolution accurate mass measurements of the precursor and fragment ions of the toxins and, their natural isotopic compounds give a higher degree of confidence in the detection of toxins (especially isobaric toxins), which often occur at very low levels in seafood samples. These methods were applied to a variety of samples; puffer fish and trumpet shell, phytoplankton, sea water and solid phase absorption toxin tracking (SPATT) samples collected from the European region. The extraction protocols were also validated taking into account matrix ion suppression effects. The methods presented in this thesis have the potential to be used as reference methods for the official control and assessment of new and emerging toxin groups - (Abstract) |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis |
| Degree type |
|
| Name of granting institution |
Cork Institute of Technology, |
| Year degree granted |
2017 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
39547 |
| Topical term or geographic name entry element |
Marine toxins |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
123927 |
| Topical term or geographic name entry element |
Tetrodotoxin |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
39280 |
| Topical term or geographic name entry element |
Liquid chromatography |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
39608 |
| Topical term or geographic name entry element |
Mass spectrometry |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
37525 |
| Topical term or geographic name entry element |
Fourier transform spectroscopy |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
123930 |
| Topical term or geographic name entry element |
Seafood industry |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |