MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03866nam a2200313 a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
2016 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
THESES PRESS |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Endersen, Lorraine, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Exploiting bacteriophages and associated peptidoglycan hydrolases with potential for biocontrol in food related applications / |
| Statement of responsibility, etc. |
Lorraine Endersen. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Cork : |
| Name of producer, publisher, distributor, manufacturer |
Cork Institute of Technology, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2015. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
v, 477 pages : |
| Other physical details |
color illustrations, diagrams, graphs, tables ; |
| Dimensions |
30 cm |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
unmediated |
| Media type code |
n |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Carrier type code |
nc |
| Source |
rdacarrier |
| 490 0# - SERIES STATEMENT |
|---|
| Series statement |
Ph.D. - Biological Sciences |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cork Institute of Technology, 2015. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The work presented in this thesis describes the isolation and characterisation of novel bacteriophages, and the subsequent exploitation of their bactericidal properties against two opportunistic food related pathogens, Mycobacterium avium subsp. paratuberculosis (MAP) and Cronobacter sakazakii. MAP is a proven animal pathogen known to cause Johne's disease in cattle but has been implicated as a causative agent of Crohn's disease in humans. Despite the fact that this association has yet to be proven, significant focus has been directed towards evaluating the consequences of consuming milk contaminated with MAP. Accordingly, six mycobacteriophages were isolated and characterised in terms of temperature and pH stability, and were subsequently demonstrated both individually and in combination to be active at inhibiting the growth of a variety of bovine MAP isolates, to varying degrees. In addition, the anti-mycobacterial efficacy of these phages was also demonstrated against the faster growing, non pathogenic surrogate strain, M. smegmatis, in reconstituted skim milk. Cronobacter sakazakii is an opportunistic foodborne pathogen that is responsible for causing necrotising enterocolitis, meningitis and bacteraemia in low birth weight infant and neonates with high mortality. In recent years, the microbiological safety of powdered infant formula has become the focus of much scientific research due to the identification of contaminating C. sakazakii and its epidemiological link with life threatening neonatal infections. In response to this, Cronobacter phage vB_CsaP_Ss1 was isolated from soil and its genome was sequenced and annotated which resulted in the identification of a putative endolysin that was subsequently cloned, expressed, purified and demonstrated to be effective at hydrolysing the peptidoglycan of C. sakazakii and other Gram-negative food related pathogens. Three additional phages were also isolated, characterised and demonstrated in combination, to be effective at controlling C. sakazakii in different brands of infant formula. Furthermore, the anti-biofilm properties of the combined phage preparation was also demonstrated. The remaining work in this thesis focused on harnessing a bacterial-derived peptidoglycan hydrolase from C. sakazakii for biocontrol of this infant formula pathogen and other related Gram-negative bacteria. In addition, the application of a novel strategy to overcome the limitations associated with the exogenous use of peptidoglycan hydrolases against Gram-negative bacteria was also investigated. - (Author's abstract) |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Bacteriophages. |
| 9 (RLIN) |
52169 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Mycobacterium avium paratuberculosis. |
| 9 (RLIN) |
93631 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enterobacter sakazakii. |
| 9 (RLIN) |
107285 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Peptidoglycans |
| 9 (RLIN) |
118288 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Pathogenic microorganisms. |
| 9 (RLIN) |
39908 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Infant formulas |
| General subdivision |
Contamination. |
| 9 (RLIN) |
118273 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Gram-negative bacteria. |
| 9 (RLIN) |
37876 |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) |
|---|
| a |
.b11192598 |
| b |
160422 |
| c |
160419 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Suppress in OPAC |
0 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) |
|---|
| a |
c |
| Operator's initials, OID (RLIN) |
|
| Cataloger's initials, CIN (RLIN) |
m |
| First Date, FD (RLIN) |
a |
| Local |
- |
| -- |
eng |
| -- |
ie |
| h |
0 |