MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02730nam a2200265 a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
2016 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
THESES PRESS |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mannion, David, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Development and validation of gas chromatographic methods for free fatty acid determination in dairy products / |
| Statement of responsibility, etc. |
David Mannion. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Cork : |
| Name of producer, publisher, distributor, manufacturer |
Cork Institute of Technology, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2016. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
iv, 152 pages : |
| Other physical details |
color illustrations, graphs, diagrams, tables ; |
| Dimensions |
30 cm |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
unmediated |
| Media type code |
n |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Carrier type code |
nc |
| Source |
rdacarrier |
| 490 0# - SERIES STATEMENT |
|---|
| Series statement |
M.Sc. - Physical Science |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cork Institute of Technology, 2016. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Quantification of free fatty acids (FFA) in dairy products by gas chromatography flame ionization detection is an important requirement for quality, research, nutritional, authenticity and legislative purposes. Common procedures are typically variants of either the fatty acid methyl esterification (FAME) or direct on-column injection methods developed more than two decades ago. This study involved the development of an alternative novel derivatization method using butylation, incorporating automation reducing solvent usage that is applicable for a wide range of dairy products. The accuracy, precision, linearity, limits of detection and limits of quantification of all three methods were assessed for the quantification of FFA in seventeen dairy products. Significant limitations were evident with both the direct on-column and FAME methods. The direct on-column method experienced accumulative column phase deterioration and irreversible FFA absorption due to the acidic nature of the injected extract. The FAME method experienced issues with co-elution of the injection solvent peak with butyric acid that impacted on its quantification, loss of polyunsaturated free fatty acids and the interference from artefact peaks. Quantification of all FFA was achieved across all samples with the novel derivatization method. The novel butylated derivatization step was automated, reducing solvent usage and increasing sample throughput and accuracy. Limites of quantification and detection were comparable to the other methods and the method has significant potential for research and industrial application. - (Author's abstract) |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Fatty Acids |
| General subdivision |
Analysis. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Dairy products |
| General subdivision |
Analysis and examination. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Gas chromatography. |
| 9 (RLIN) |
37689 |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) |
|---|
| a |
.b11192513 |
| b |
160418 |
| c |
160414 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Suppress in OPAC |
0 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) |
|---|
| a |
c |
| Operator's initials, OID (RLIN) |
160414 |
| Cataloger's initials, CIN (RLIN) |
m |
| First Date, FD (RLIN) |
a |
| Local |
- |
| -- |
eng |
| -- |
ie |
| h |
0 |