MTU Cork Library Catalogue

Recombinant DNA and biotechnology : a guide for students /

Kreuzer, Helen.

Recombinant DNA and biotechnology : a guide for students / Helen Kreuzer and Adrianne Massey. - 2nd ed. - Washington, D.C. : ASM Press, c2001. - xiv, 431 p., [3] p. of plates : ill. (some col.), maps ; 28 cm. + pbk.

Includes bibliographical references and index.

Part I Laying the foundation: An overview of biotechnology -- Biotechnology and society -- Genes, genetics and geneticists -- An overview of molecular biology -- Applying molecular biology: recombinant DNA technology -- Part II Classroom activities: A. DNA structure and function: Constructing a paper helix -- DNA polymerase: the replicator -- From genes to proteins, Does antisense make sense? -- Sizes of the Escherichia coli and human genomes -- Extraction of bacterial DNA -- B. Manipulating and analysis of DNA: DNA scissors -- DNA goes to the races -- Restriction analysis of lambda DNA -- Recombinant paper plasmids -- Restriction analysis challenge -- Detection of specific DNA sequences, Part I. Fishing for DNA, Part II. Combining restriction and hybridization, Part III. Southern hybridization -- DNA sequencing: the terminators, Reading: Chain terminators as antiviral drugs -- The polymerase chain reaction: paper PCR -- Analyzing genetic variation: DNA typing, Reading: Mitochondrial DNA in DNA typing -- A mix-up at the hospital -- A paternity case -- The case of the bloody knife -- C. Transfer of genetic information: Transformation of Escherichia coli -- Conjugative transfer of antibiotic resistance in Escherichia coli, Reading: Gene transfer in nature -- Transduction of an antibiotic resistance gene -- Agrobacterium tumefaciens: nature's plant genetic engineer -- D. Bioinformatics and evolutionary analysis of proteins: Testing for amylase activity -- Electrophoresis of amylase samples -- Constructing an amylase evolutionary tree -- Bioinformatics -- E. Molecular biology and genetics: An adventure in dog hair, Part I -- An adventure in dog hair, Part II: yellow labs -- Human molecular genetics, Reading: the human genome project: its science, applications, and issues, Reading: Molecular genetics of cancer -- Part III Societal issues: Introduction -- Science, technology and society -- Weighing technology's risks and benefits, Reading: Are bioengineered foods safe? -- Debating the risks of biotechnology -- A decision-making model for biothical issues -- Bioethics case study: gene therapy -- Bioethics case study: genetic screening -- Careers in biotechnology.

1555811760

2001273152


Recombinant DNA.
Genetic engineering.
Biotechnology

575.1

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